A RESEARCH OF PROTEINS AND PEPTIDES IN DRONE BROOD
LARVAE AT DIFFERENT STAGES OF DEVELOPMENT

Grishina Zhanna Waler’evna, Postrgraduate student, Penza State University (40 Krasnaya street, Penza, Russia), grinzanetk@gmail.com
Gengin Mikhail Trofimovich, Doctor of biological sciences, professor, sub-department of biology and biochemistry, Penza State University (40 Krasnaya street, Penza, Russia), gengin07@yandex.ru

577.12

10.21685/2307-9150-2016-3-6

Background. The physical, chemical and biological properties of proteins and peptides in ontogeny of drone larvae were researched. The dynamics of changes of proteins and peptides in drone brood larvae at different stages of development was analyzed.
Materials and methods. The experiment was carried out in drone brood larvae. Extracts of peptides were prepared by protein precipitation of 5 % TCA. The dy-namics of changes of proteins and peptides was investigated by the gel filtration method.
Results. The authors have ascertained the dynamics of changes of proteins and peptides in drone brood larvae at different stages of development. The article shows the quantitative and qualitative differentiation of proteins and peptides by larvae age. It has been found that the content of peptides peaks on the 5th day of larval development, the minimum is observed on the 8th day.
Conclusions. It is assumed that the content of such dynamics of peptides at various stages of development of drone larvae is caused by different intensity of processes, partially regulated by peptides.

proteins and peptides, drone brrod larvae, gel filtration method.

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